13- STUDY THE EFFECT OF DIFFERENT CULTURE MEDIA ON SOME FROZEN SEMEN CHARACTERISTICS OF IRAQI BLACK LOCAL GOAT SPERMATOZOA
Basrah Journal of Veterinary Research.,
Volume 17, Issue 1, Pages 147-154
AbstractThe semen was collected from five adult Iraqi black local bucks by the
artificial vagina and using estrous induced doe the semen was diluted with egg yolk
10% extender and frozen by liquid nitrogen for three months, macroscopic and
microscopic evaluation were done after collection, dilution and thawing, three
media(TCM-199, DMEM and SOF)were used to see the their effect on activation of
spermatozoa. The results showed that TCM-199 media presented higher significance
in sperm motility, progressive motility compared with DMEM and SOF media.
There was a great decrease in the concentration of spermatozoa after activation using
these media which didn’t differ significantly among them and they were higher
significantly compared with control in normal morphology.
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